|Room 401, Section 2, Bld 25, Keyuanxi Industrial Park, Nanshan District, Shenzhen City GD, China|
Payment & Shipping Terms:
|Specification:||40T/Kit||Shelf Life:||12 Months|
|Storage Temperature:||2 - 8℃||Test Sample:||Semen Plasma|
Male Infertility Diagnostic Kit for Determination of the Maturity of Spermatozoan Nucleoprotein Aniline Blue Staining
In normal circumstances, the basic protein (nucleoprotein) bound to the sperm nucleus DNA will undergo the process of natural maturity from the histone to protamine, of which, such substitute process of histone by protamine is called nucleoprotein transition, which has an important physiological significance. The spermatozoid nucleus carries all genetic information from paternal, and these genes must start the expression after the fertilization. Before the fertilization, the sperm genes are specially protected by the protamine and closely concentrate with no DNA transcription. However, the abnormality of nucleoprotein transition may cause the male infertility or early embryo abortion. The mechanism lies in: a) the instability in sperm DNA and easily to be damaged and difficult to get pregnant; b) once fertilized, the spermatozoid nucleus cannot normally depolymerized due to the nucleoprotein pattern, and thereby to influence the binding of female and male pronuclei; c) the abnormal development of embryo may induce to the embryo abortion.
This kit is designed for semi-quantitative detection of maturity of human spermatozoan nucleoprotein.
Aniline blue staining method: The nucleoprotein of mature sperm is composed of protamine containing arginine and cysteine residue; the nucleoprotein composition of immature sperm is histone, which contains lysine residue. In the acidic condition, the aniline blue binds to the lysine residues and produce the dark blue compound, and thus to indicate the existence of immature nucleoprotein, while the sperm with mature nucleoprotein can stained red.
The sketch map of test result:
Contact Person: Cindy