• Kit to Evaluate Sperm DNA Integrity
  • Kit to Evaluate Sperm DNA Integrity
  • Kit to Evaluate Sperm DNA Integrity
Kit to Evaluate Sperm DNA Integrity

Kit to Evaluate Sperm DNA Integrity

Product Details:

Place of Origin: China
Brand Name: BRED
Certification: CE, ISO13485
Model Number: BRED-029

Payment & Shipping Terms:

Minimum Order Quantity: 1 kits
Price: Negotiable
Packaging Details: 20T/Kit
Delivery Time: 3 - 5 working days
Payment Terms: T/T,
Supply Ability: 10000 kits per year
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Detail Information

Shelf Life: 12 Months Storage Temperature: 2 - 8 ℃
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Product Description

Sperm DNA Fragmentation Sperm Chromatin Structure Assay Kit AOT

Flow Cell Methodology


Packing specification: 

  • 20 tests/kit

Intended use:

  • The kit is designed to evaluate the DNA integrity and maturity of human sperm by staining sperm chromatin.

Test principle:


After denatured by acid, fragmented sperm chromatin would turn to single strand. And the normal sperm chromatin retain double strand. Acridine Orange(AO) associated with single-stranded DNA emits red or yellow fluorescence at 488nm luminescence. While AO intercalated in double stranded DNA emits green fluorescene. If the red fluorescence is increasing, it shows a high rate of DNA fragmentation in sperms.


Main compositions:

  • Reagent A 1 vial 2.5 ml
  • Reagent B 1 vial 4.5 ml
  • Reagent C ( lyophilized powder ) 2 vials 13 ml 6.5ml/vial
  • Lyophilized powder dissolved solution 1 vial 4 ml

Storage conditions and duration:

  • Storage conditions: The kit is kept at 2 ~ 8 ℃ in dark place.
  • Period of validity: The kit is kept at 2 ~ 8 ℃ for one year. After dissolved, the reagent C would be kept for two weeks in dark at 2 ~ 8 ℃. Once separately store the dissolved reagent C at -20 ℃ in dark, the use life of reagent C could be more than one year.

Applicable instrument:

  • Flow cytometer (488nm luminescence)

Specimen requirements:

  • The sample should be collected after a minimum of 48 hours but not longer than seven days of sexual abstinency by masturbation or sexual intercourse using special condoms. The semen sample must be complete in collection.
  • Fresh semen samples should be liquefied completely prior to testing. If the samples have delayed liquefaction or high viscosity, a liquefier (sold separately) should be conducted.

Kit to Evaluate Sperm DNA Integrity 0

Kit to Evaluate Sperm DNA Integrity 1

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