37 ℃ Water Bath Seminal Reactive Oxygen Species NBT Staining Kit

Rapid test for sperm superoxide anion (O₂^-.)

Seminal Reactive Oxygen Species NBT Staining Kit

Applications: 

• Analyze the etiology of infertility.
• Useful supplements for routine semen analysis,
• can be carried out routinely.
• Patient ROS level detection and efficacy monitoring after antioxidant treatment.
• Monitor the change of seminal ROS, and select the optimal sperm for fertilization.
• Analyze the reasons of ART adverse outcome.
• Analyze the reasons of low quality semen parameters

Features:

• The test process is simple, no special equipment required.
• Easy semi-quantitative detection with a small amount of semen.
• Required equipment: ordinary 37 ℃ water bath.

Test process:

Test results:

The NBT test method is adopted in the form of chromogenic reactions, and the chromogenic product is derived from the formazan, the reductive substance of tetrazole.Semi-quantitative detection of ROS (O₂⁻ •) is based on the color of formazan.

Diagram of formazan deposit by NBT reaction of Sperm/leukocyte ROS

(a) Negative sperm; (b) Sperm with positive head;

(c) Sperm with positive midpiece; (d) Sperm with positive acrosome;

(e) Weakly positive leukocyte; (f) Strongly positive leukocyte.